Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Aspergillus spp. has been found worldwide in almost all domestic and wild animals, ranging from insects and corals to NHP. Aspergillosis is a common infection in birds, particularly pet parrots, penguins, captive raptors, mallards and other ducks, turkeys, and Japanese quail, in which it causes pulmonary and air sac infection. Aspergillosis has also been described in cats and dogs in which it affects only the nasal passages. In large animals, Aspergillus infection is assumed to be rare but has been reported with increasing frequency. It can lead to various diseases like mycotic abortion and gland infection in cows as well as guttural pouch involvement in horses. In marine mammals, airways are usually initially affected leading to pneumonia, but other organs including the brain may also be infected following fatal dissemination. The most frequently identified pathogens are Aspergillus fumigatus and Aspergillus flavus, ubiquitous organisms capable of living under extensive environmental stress.
Aspergillus fumigatus is an opportunistic pathogen and the primary cause of invasive aspergillosis. Triazole antifungals are used to treat aspergillosis. However, triazole-resistant A. fumigatus infections are increasingly reported worldwide and are associated with increased treatment failure and mortality. This fungus can acquire resistance to azole antifungals due to mutations in the azole target (Cyp51A). Cyp51A mutations typical for environmental azole resistance acquisition (for example, TR34/L98H) have been reported in many cases. These mutations can also be found in isolates recovered from patients.
Pathogens Tested
APA-098 :Aspergillus fumigatus/ terreus/ flavus
APA-189 : Azole-resistance profiling of Cyp51A gene
Method
Aspergillus fumigatus / terreus / flavus by Multi Real-Time PCR (This assay includes the detection and differentiation of Aspergillus fumigatus / terreus / flavus) Azole-resistance profiling of Cyp51A gene(This assay detects selected mutations in the Cyp51A gene related to azole-resistance, by PCR and sequencing).
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Histoplasma capsulatum is an ascomycetous fungus and belongs to the fungal family Ajellomycetaceae. It causes Histoplasmosis which is a type of lung infection caused by inhaling the fungal spores of Histoplasma capsulatum . Histoplasma capsulatum is dimorphic and switches from a mould-like (filamentous) growth form in the natural habitat to a small budding yeast form in the warm-blooded animal host. Histoplasma capsulatum appears to be strongly associated with the droppings of certain bird species as well as bats. A mixture of these droppings and certain soil types is particularly conducive to proliferation. In highly endemic areas there is a strong association with soil under and around chicken houses, and with areas where soil or vegetation has become heavily contaminated with faecal material deposited by flocking birds such as starlings and blackbirds.
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Aspergillus spp. has been found worldwide in almost all domestic and wild animals, ranging from insects and corals to NHP. Aspergillosis is a common infection in birds, particularly pet parrots, penguins, captive raptors, mallards and other ducks, turkeys, and Japanese quail, in which it causes pulmonary and air sac infection. Aspergillosis has also been described in cats and dogs in which it affects only the nasal passages. In large animals, Aspergillus infection is assumed to be rare but has been reported with increasing frequency. It can lead to various diseases like mycotic abortion and gland infection in cows as well as guttural pouch involvement in horses. In marine mammals, airways are usually initially affected leading to pneumonia, but other organs including the brain may also be infected following fatal dissemination. The most frequently identified pathogens are Aspergillus fumigatus and Aspergillus flavus, ubiquitous organisms capable of living under extensive environmental stress.
Aspergillus fumigatus is an opportunistic pathogen and the primary cause of invasive aspergillosis. Triazole antifungals are used to treat aspergillosis. However, triazole-resistant A. fumigatus infections are increasingly reported worldwide and are associated with increased treatment failure and mortality. This fungus can acquire resistance to azole antifungals due to mutations in the azole target (Cyp51A). Cyp51A mutations typical for environmental azole resistance acquisition (for example, TR34/L98H) have been reported in many cases. These mutations can also be found in isolates recovered from patients.
Pathogens Tested
APA-098 :Aspergillus fumigatus/ terreus/ flavus
APA-189 : Azole-resistance profiling of Cyp51A gene
Method
Aspergillus fumigatus / terreus / flavus by Multi Real-Time PCR (This assay includes the detection and differentiation of Aspergillus fumigatus / terreus / flavus) Azole-resistance profiling of Cyp51A gene(This assay detects selected mutations in the Cyp51A gene related to azole-resistance, by PCR and sequencing).
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Histoplasma capsulatum is an ascomycetous fungus and belongs to the fungal family Ajellomycetaceae. It causes Histoplasmosis which is a type of lung infection caused by inhaling the fungal spores of Histoplasma capsulatum . Histoplasma capsulatum is dimorphic and switches from a mould-like (filamentous) growth form in the natural habitat to a small budding yeast form in the warm-blooded animal host. Histoplasma capsulatum appears to be strongly associated with the droppings of certain bird species as well as bats. A mixture of these droppings and certain soil types is particularly conducive to proliferation. In highly endemic areas there is a strong association with soil under and around chicken houses, and with areas where soil or vegetation has become heavily contaminated with faecal material deposited by flocking birds such as starlings and blackbirds.
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
